The surface of the tooth is covered by a biofilm of microorganism, especially bacteria. Tooth decay or dental caries results from the acids secreted by certain bacteria. Streptococcus mutans are the common type of acid-producing oral pathogen that is present mainly in the mouth, pharynx and intestine. It decays the tooth by secreting glucose polysaccharides known as glucans.
LactoSporin® was evaluated for the effect of oral hygiene by assessing its impact on the growth and drop in pH level of S. mutans.
A significant reduction (for about 12 hours) in the growth, rate of pH drop (prevention from detrimental acidic environment) and glucan production was observed in S. mutans within 5 minutes of the LactoSporin® treatment. Thus, suggesting the role of LactoSporin® in oral hygiene.
Inhibition of pH drop
Water insoluble glucan inhibition
Prevention of pH drop and glucan production by LactoSporin®
Biofilm inhibitory potential of LactoSporin®
Biofilm, the extracellular matrix on living or non-living surfaces are less susceptible to antimicrobial agents. Thus, these associations of microbial cells are difficult to target and treat.
Biofilm inhibitory potential of LactoSporin® was evaluated by using an antimicrobial test known as broth micro-dilution method.
LactoSporin® exhibited a concentration-dependent biofilm inhibitory effect against S. mutans, S. aureus, P. aeruginosa and E. Coli.
S. mutans MTCC 1943
P. aeruginosa ATCC 9027
S. aureus ATCC 29213
E. Coli ATCC 25922
Biofilm inhibitory effect of LactoSporin®